产品描述
This Human NPTX1 ELISA Kit was designed for the quantitative measurement of Human NPTX1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Neuronal pentraxin I ELISA Kit; Neuronal pentraxin-1 ELISA Kit; NP 1 ELISA Kit; NP-I ELISA Kit; NP1 ELISA Kit; Nptx1 ELISA Kit; NPTX1_HUMAN ELISA Kit
缩写
NPTX1
Uniprot No
Q15818
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human NPTX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-97 | |
| 1:2 | Average % | 103 | |
| Range % | 96-105 | |
| 1:4 | Average % | 93 | |
| Range % | 89-97 | |
| 1:8 | Average % | 98 | |
| Range % | 94-102 | |
回收率
| The recovery of human NPTX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-101 | |
| EDTA plasma (n=4) | 92 | 86-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.642 | 2.443 | 2.543 | 2.395 | | | 250 | 1.838 | 1.737 | 1.788 | 1.640 | | | 125 | 1.034 | 1.166 | 1.100 | 0.952 | | | 62.5 | 0.613 | 0.597 | 0.605 | 0.457 | | | 31.2 | 0.398 | 0.401 | 0.400 | 0.252 | | | 15.6 | 0.264 | 0.255 | 0.260 | 0.112 | | | 7.8 | 0.196 | 0.188 | 0.192 | 0.044 | | | 0 | 0.149 | 0.147 | 0.148 | | |
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货期
3-5 working days