产品描述

The human interleukin-36 gamma (IL36γ) ELISA kit is suitable for quantitatively determining the human IL36γ complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36γ levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

IL36γ, also called IL1F9, is an IL-1 family cytokine involved in polarizing Type-1 immune responses. IL36G can be induced in keratinocytes, bronchial epithelia, brain tissues, and macrophages and is regarded as an "alarmin" in damaged tissue. Chenyang Dai et al. demonstrated that the IL-36γ/IL-36R axis plays a protective role in Candida albicans (CA) keratitis through upregulating AMPs and downregulating the inflammatory response and consequently improves the outcome of CA keratitis.

别名

IL 1 epsilon ELISA Kit; IL 1 related protein 2 ELISA Kit; IL 1(EPSILON) ELISA Kit; IL 1F9 ELISA Kit; IL 1H1 ELISA Kit; IL 1RP2 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1-related protein 2 ELISA Kit; IL-1F9 ELISA Kit; IL-1H1 ELISA Kit; IL-1RP2 ELISA Kit; IL1E ELISA Kit; Il1f9 ELISA Kit; IL1F9_HUMAN ELISA Kit; IL1H1 ELISA Kit; IL1RP2 ELISA Kit; IL36 gamma ELISA Kit; IL36G ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 9 ELISA Kit; Interleukin 1 homolog 1 ELISA Kit; Interleukin 1 related protein 2 ELISA Kit; Interleukin 36 gamma ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 9 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit

缩写

IL1F9

Uniprot No

Q9NZH8

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		82-95
1:2		Average %		96
		Range %		90-101
1:4		Average %		97
		Range %		92-106
1:8		Average %		95
		Range %		85-99

回收率

The recovery of human IL1F9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		86-98
EDTA plasma (n=4)		92		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 5000 2.052 1.952 2.002 1.898 2500 1.611 1.497 1.554 1.450 1250 1.242 1.204 1.223 1.119 625 0.814 0.757 0.786 0.682 312 0.583 0.538 0.561 0.457 156 0.313 0.289 0.301 0.197 78 0.201 0.199 0.200 0.096 0 0.107 0.101 0.104

货期

3-5 working days