产品描述
This Human IL7R ELISA Kit was designed for the quantitative measurement of Human IL7R protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .
别名
IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127
缩写
IL7R
Uniprot No
P16871
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human sIL-7R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 87-93 | |
| 1:2 | Average % | 103 | |
| Range % | 99-105 | |
| 1:4 | Average % | 90 | |
| Range % | 85-93 | |
| 1:8 | Average % | 99 | |
| Range % | 94-103 | |
回收率
| The recovery of human sIL-7R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 90-95 | |
| EDTA plasma (n=4) | 96 | 92-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.525 | 2.500 | 2.513 | 2.376 | | | 25 | 1.940 | 1.894 | 1.917 | 1.780 | | | 12.5 | 1.287 | 1.267 | 1.277 | 1.140 | | | 6.25 | 0.701 | 0.678 | 0.690 | 0.553 | | | 3.12 | 0.444 | 0.448 | 0.446 | 0.309 | | | 1.56 | 0.323 | 0.314 | 0.319 | 0.182 | | | 0.78 | 0.231 | 0.222 | 0.227 | 0.090 | | | 0 | 0.139 | 0.135 | 0.137 | | |
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货期
3-5 working days