产品描述
IL-22(Interleukin-22),属于IL-10家族细胞因子,是由激活的T细胞(特别是Th17细胞)和NK细胞产生的。IL-22受体表达在各种细胞上,包括上皮细胞、免疫细胞和肝细胞等。IL-22具有促进上皮细胞增殖和保护上皮细胞免受损伤的作用,同时在炎症和肿瘤发生中也扮演了重要角色。
华美生物所提供的Mouse Interleukin 22(IL-22) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的IL22,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
Il22 ELISA Kit; Il22a ELISA Kit; Iltif ELISA Kit; IltifaInterleukin-22 ELISA Kit; IL-22 ELISA Kit; IL-10-related T-cell-derived-inducible factor ELISA Kit; IL-TIF ELISA Kit; IL-TIF alpha ELISA Kit; Interleukin-22a ELISA Kit; IL-22a ELISA Kit
缩写
IL22
Uniprot No
Q9JJY9
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
106 |
|
| Range % |
101-113 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
92-103 |
|
| 1:4 |
Average % |
90 |
|
| Range % |
85-94 |
|
| 1:8 |
Average % |
94 |
|
| Range % |
90-98 |
|
回收率
| The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-100 |
|
| EDTA plasma (n=4) |
89 |
84-93 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.309 |
2.438 |
2.374 |
2.157 |
|
| 10 |
1.628 |
1.729 |
1.679 |
1.462 |
|
| 5 |
1.193 |
1.221 |
1.207 |
0.990 |
|
| 2.5 |
0.824 |
0.805 |
0.815 |
0.598 |
|
| 1.25 |
0.605 |
0.627 |
0.616 |
0.399 |
|
| 0.625 |
0.462 |
0.450 |
0.456 |
0.239 |
|
| 0.312 |
0.327 |
0.312 |
0.320 |
0.103 |
|
| 0 |
0.220 |
0.214 |
0.217 |
|
|
|
货期
3-5 working days