产品描述

This Human L1CAM ELISA Kit was designed for the quantitative measurement of Human L1CAM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.31 ng/mL.

别名

Antigen identified by monoclonal ELISA Kit R1 ELISA Kit; CAML1 ELISA Kit; CD171 ELISA Kit; CD171 antigen ELISA Kit; HSAS ELISA Kit; HSAS1 ELISA Kit; Hyd ELISA Kit; L1 ELISA Kit; L1 cell adhesion molecule ELISA Kit; L1-NCAM ELISA Kit; L1cam ELISA Kit; L1CAM_HUMAN ELISA Kit; MASA ELISA Kit; MIC5 ELISA Kit; N CAML1 ELISA Kit; N-CAM-L1 ELISA Kit; NCAM-L1 ELISA Kit; NCAML1 ELISA Kit; Nerve-growth factor-inducible large external glycoprotein ELISA Kit; Neural cell adhesion molecule L1 ELISA Kit; NILE ELISA Kit; OTTHUMP00000025992 ELISA Kit; S10 ELISA Kit; SPG1 ELISA Kit

缩写

L1CAM

Uniprot No

P32004

灵敏度

0.31 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NCAM-L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		86-92
1:2		Average %		107
		Range %		99-111
1:4		Average %		89
		Range %		85-93
1:8		Average %		99
		Range %		92-103

回收率

The recovery of human NCAM-L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		84-95
EDTA plasma (n=4)		104		95-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 80 2.014 1.994 2.004 1.902 40 1.429 1.405 1.417 1.315 20 0.863 0.844 0.854 0.752 10 0.477 0.465 0.471 0.369 5 0.281 0.287 0.284 0.182 2.5 0.225 0.233 0.229 0.127 1.25 0.156 0.152 0.154 0.052 0 0.103 0.101 0.102

货期

3-5 working days