产品描述
The Human Plasmin-Antiplasmin Complex (PAP) ELISA Kit is a highly sensitive and reliable tool for the quantitative detection of PAP in various sample types. PAP is a complex of plasmin and its primary inhibitor, antiplasmin, which plays a critical role in regulating fibrinolysis, a process involved in blood clotting and dissolution.
This ELISA kit can be used to detect human PAP in serum, plasma, cell culture supernates, and urine. Based on a sandwich assay principle, our kit provides accurate and reproducible results with minimal interference from other proteins.
With a sensitivity of 0.039 ng/mL and a detection range of 0.156 ng/mL-10 ng/mL, our ELISA kit can accurately measure PAP levels in various sample types. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PAP levels. With its high sensitivity, wide detection range, and reliable results, this ELISA kit is an excellent choice for researchers looking to gain deeper insights into the role of PAP in human health and disease.
别名
N/A
缩写
PAP
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 88 | |
| Range % | 80-92 | |
| 1:200 | Average % | 100 | |
| Range % | 91-108 | |
| 1:400 | Average % | 100 | |
| Range % | 92-109 | |
| 1:800 | Average % | 92 | |
| Range % | 86-100 | |
回收率
| The recovery of human PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 89-101 | |
| EDTA plasma (n=4) | 93 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.819 | 2.815 | 2.817 | 2.688 | | | 5 | 2.244 | 2.250 | 2.247 | 2.118 | | | 2.5 | 1.291 | 1.301 | 1.296 | 1.167 | | | 1.25 | 0.730 | 0.793 | 0.762 | 0.633 | | | 0.625 | 0.443 | 0.453 | 0.448 | 0.319 | | | 0.312 | 0.231 | 0.250 | 0.241 | 0.112 | | | 0.156 | 0.157 | 0.168 | 0.163 | 0.034 | | | 0 | 0.124 | 0.134 | 0.129 | | |
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货期
3-5 working days
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