产品描述
This Pig IL17A ELISA Kit was designed for the quantitative measurement of Pig IL17A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 20 pg/mL- 800 pg/mL and the sensitivity is 10 pg/mL.
别名
IL17A ELISA Kit; IL17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit
缩写
IL17A
Uniprot No
Q60I29
灵敏度
10 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-96 |
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| 1:2 |
Average % |
99 |
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| Range % |
92-106 |
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| 1:4 |
Average % |
89 |
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| Range % |
83-94 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-98 |
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回收率
| The recovery of pig IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-99 |
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| EDTA plasma (n=4) |
84 |
80-88 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 800 |
2.806 |
2.862 |
2.834 |
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| 400 |
2.080 |
2.097 |
2.089 |
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| 200 |
1.174 |
1.198 |
1.186 |
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| 100 |
0.698 |
0.663 |
0.681 |
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| 40 |
0.281 |
0.294 |
0.288 |
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| 20 |
0.147 |
0.145 |
0.146 |
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货期
3-5 working days