产品描述
This Mouse LTA ELISA Kit was designed for the quantitative measurement of Mouse LTA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.
别名
Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
缩写
LTA
Uniprot No
P09225
灵敏度
4.7 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-100 | |
| 1:2 | Average % | 105 | |
| Range % | 100-110 | |
| 1:4 | Average % | 92 | |
| Range % | 88-100 | |
| 1:8 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of mouse TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 90 | 85-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1200 | 2.674 | 2.752 | 2.713 | 2.534 | | | 600 | 2.254 | 2.176 | 2.215 | 2.036 | | | 300 | 1.435 | 1.404 | 1.420 | 1.241 | | | 150 | 0.805 | 0.839 | 0.822 | 0.643 | | | 75 | 0.498 | 0.524 | 0.511 | 0.332 | | | 37.5 | 0.356 | 0.338 | 0.347 | 0.168 | | | 18.75 | 0.254 | 0.239 | 0.247 | 0.068 | | | 0 | 0.175 | 0.182 | 0.179 | | |
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货期
3-5 working days