产品描述
This Mouse LT-B4 ELISA Kit was designed for the quantitative measurement of Mouse LT-B4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
N/A
缩写
LT-B4
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-104 | |
| 1:2 | Average % | 91 | |
| Range % | 86-97 | |
| 1:4 | Average % | 95 | |
| Range % | 91-99 | |
| 1:8 | Average % | 95 | |
| Range % | 89-99 | |
回收率
| The recovery of mouse LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-101 | |
| EDTA plasma (n=4) | 93 | 89-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.334 | 2.256 | 2.295 | 2.130 | | | 500 | 1.876 | 1.934 | 1.905 | 1.740 | | | 250 | 1.575 | 1.517 | 1.546 | 1.381 | | | 125 | 1.176 | 1.089 | 1.133 | 0.968 | | | 62.5 | 0.697 | 0.733 | 0.715 | 0.550 | | | 31.2 | 0.468 | 0.438 | 0.453 | 0.288 | | | 15.6 | 0.294 | 0.287 | 0.291 | 0.126 | | | 0 | 0.160 | 0.169 | 0.165 | | |
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货期
3-5 working days