产品描述

This Human IL1F8 ELISA Kit was designed for the quantitative measurement of Human IL1F8 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

别名

2310043N20Rik ELISA Kit; Family of interleukin 1 eta ELISA Kit; FIL1 (ETA) ELISA Kit; FIL1 ELISA Kit; FIL1 eta ELISA Kit; FIL1H ELISA Kit; FILI-(ETA) ELISA Kit; IL 1F8 (FIL1 eta ELISA Kit; IL 1F8 ELISA Kit; IL 1H2 ELISA Kit; IL-1 eta ELISA Kit; IL-1F8 ELISA Kit; IL-1H2 ELISA Kit; IL1 ETA ELISA Kit; IL1F8 (Canonical product IL 1F8a) ELISA Kit; IL1F8 ELISA Kit; IL1F8_HUMAN ELISA Kit; IL1H2 ELISA Kit; IL36 beta ELISA Kit; IL36B ELISA Kit; Interleukin 1 family, member 8 (eta) ELISA Kit; Interleukin 1 family, member 8 ELISA Kit; Interleukin 1 homolog 2 ELISA Kit; Interleukin 1 Superfamily e ELISA Kit; Interleukin 36 beta ELISA Kit; Interleukin-1 eta ELISA Kit; Interleukin-1 family member 8 ELISA Kit; Interleukin-1 homolog 2 ELISA Kit; MGC126880 ELISA Kit; MGC126882 ELISA Kit; OTTMUSP00000012797 ELISA Kit; RP23-176J12.1 ELISA Kit

缩写

IL1F8

Uniprot No

Q9NZH7

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		84-92
1:2		Average %		96
		Range %		90-102
1:4		Average %		89
		Range %		85-94
1:8		Average %		88
		Range %		84-95

回收率

The recovery of human IL1F8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		86		80-92
EDTA plasma (n=4)		100		96-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.537 2.598 2.568 2.404 500 2.062 2.091 2.077 1.913 250 1.399 1.368 1.384 1.220 125 0.799 0.771 0.785 0.621 62.5 0.555 0.534 0.545 0.381 31.2 0.374 0.395 0.385 0.221 15.6 0.271 0.294 0.283 0.119 0 0.163 0.165 0.164

货期

3-5 working days