产品描述
This Pig IL12/P70 ELISA Kit was designed for the quantitative measurement of Pig IL12/P70 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL .
别名
IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
缩写
IL-12/P70
Uniprot No
Q29053
灵敏度
0.11 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-96 | |
| 1:2 | Average % | 96 | |
| Range % | 91-101 | |
| 1:4 | Average % | 84 | |
| Range % | 80-89 | |
| 1:8 | Average % | 93 | |
| Range % | 88-97 | |
回收率
| The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 86-95 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 30 | 2.549 | 2.452 | 2.501 | 2.331 | | | 15 | 1.789 | 1.761 | 1.775 | 1.605 | | | 7.5 | 1.025 | 1.004 | 1.015 | 0.845 | | | 3.75 | 0.591 | 0.549 | 0.570 | 0.400 | | | 1.8 | 0.402 | 0.374 | 0.388 | 0.218 | | | 0.9 | 0.321 | 0.309 | 0.315 | 0.145 | | | 0.45 | 0.258 | 0.245 | 0.252 | 0.082 | | | 0 | 0.173 | 0.167 | 0.170 | | |
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货期
3-5 working days