产品描述
IL6(白细胞介素6, interleukin-6)是一种细胞因子,参与多种免疫和炎症反应。在感染和炎症等情况下,IL6的水平明显升高。
华美生物所提供的Chicken Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鸡血清、血浆、组织匀浆样本中的IL6,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL6
Uniprot No
Q90YI0
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-100 |
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回收率
| The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
91-105 |
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| EDTA plasma (n=4) |
96 |
88-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.487 |
2.570 |
2.529 |
2.454 |
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| 500 |
2.204 |
2.230 |
2.217 |
2.142 |
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| 250 |
1.800 |
1.764 |
1.782 |
1.707 |
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| 125 |
1.390 |
1.394 |
1.392 |
1.317 |
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| 62.5 |
0.917 |
0.950 |
0.934 |
0.859 |
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| 31.2 |
0.584 |
0.568 |
0.576 |
0.501 |
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| 15.6 |
0.348 |
0.362 |
0.355 |
0.280 |
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| 0 |
0.074 |
0.075 |
0.075 |
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货期
3-5 working days