产品描述
This Hamster IL1B ELISA Kit was designed for the quantitative measurement of Hamster IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
缩写
IL-1β
Uniprot No
Q9WVQ9
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-98 | |
| 1:2 | Average % | 105 | |
| Range % | 100-112 | |
| 1:4 | Average % | 89 | |
| Range % | 84-93 | |
| 1:8 | Average % | 98 | |
| Range % | 94-102 | |
回收率
| The recovery of hamster IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-99 | |
| EDTA plasma (n=4) | 84 | 80-88 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.578 | 2.627 | 2.603 | 2.460 | | | 400 | 2.009 | 2.106 | 2.058 | 1.915 | | | 200 | 1.227 | 1.267 | 1.247 | 1.104 | | | 100 | 0.571 | 0.535 | 0.553 | 0.410 | | | 50 | 0.308 | 0.329 | 0.319 | 0.176 | | | 25 | 0.231 | 0.219 | 0.225 | 0.082 | | | 12.5 | 0.204 | 0.215 | 0.210 | 0.067 | | | 0 | 0.145 | 0.140 | 0.143 | | |
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货期
3-5 working days