产品描述

The human ITLN1 ELISA Kit allows for the in vitro quantitative determination of ITLN1 concentrations in serum, plasma, cell culture supernates, or tissue homogenates. This kit exclusively recognizes human ITLN1. Omentin, also called intelectin-1 (ITLN1), is an adipokine with an anti-inflammatory function and is generated by the stromal-vascular fraction of visceral adipose tissue. ITLN1 has recently been identified as a secretory and galactofuranose-binding lectin and is primarily produced in the gastrointestinal goblet cells and omentum. Evidence has shown that ITLN1 is involved in the gut immune defense against microorganisms, insulin-stimulated glucose uptake, chronic obstructive pulmonary disease, and asthma. ITLN1 also participates in neoplastic diseases such as pleural mesothelioma and gastric and prostate cancer.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. ITLN1 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled ITLN1 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with ITLN1 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

别名

Endothelial lectin HL 1 ELISA Kit; Endothelial lectin HL-1 ELISA Kit; FLJ20022 ELISA Kit; Galactofuranose binding lectin ELISA Kit; Galactofuranose-binding lectin ELISA Kit; hIntL ELISA Kit; HL 1 ELISA Kit; HL-1 ELISA Kit; HL1 ELISA Kit; Intelectin ELISA Kit; Intelectin 1 ELISA Kit; Intelectin-1 ELISA Kit; Intestinal lactoferrin receptor ELISA Kit; INTL ELISA Kit; ITLN ELISA Kit; ITLN-1 ELISA Kit; ITLN1 ELISA Kit; ITLN1_HUMAN ELISA Kit; LFR ELISA Kit; Omentin ELISA Kit; OTTHUMP00000027882 ELISA Kit

缩写

ITLN1

Uniprot No

Q8WWA0

灵敏度

0.39 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human omentin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		85
		Range %		80-92
1:400		Average %		96
		Range %		92-100
1:800		Average %		102
		Range %		93-110
1:1600		Average %		95
		Range %		82-99

回收率

The recovery of human omentin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		80-100
EDTA plasma (n=4)		96		90-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 100 2.349 2.192 2.271 2.166 50 1.869 1.806 1.838 1.733 25 1.354 1.294 1.324 1.219 12.5 0.923 0.914 0.919 0.814 6.25 0.592 0.559 0.576 0.471 3.12 0.322 0.299 0.311 0.206 1.56 0.213 0.209 0.211 0.106 0 0.109 0.101 0.105

货期

3-5 working days