产品描述
MB (肌红蛋白,Myoglobin) 是一种在肌肉细胞中广泛表达的细胞色素。MB主要参与细胞氧气传递和肌肉代谢,同时在心肌梗塞等心脏疾病的早期诊断中也发挥重要作用。在心肌细胞受损后,MB会被释放到血液中,其含量的变化可以反映心肌细胞损伤的程度和恢复情况。
华美生物所提供的Human Myoglobin,MYO/MB ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的MB,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
MB ELISA Kit; MGC13548 ELISA Kit; MYG_HUMAN ELISA Kit; Myoglobin ELISA Kit
缩写
MB
Uniprot No
P02144
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYO/MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:10 |
Average % |
92 |
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| Range % |
89-99 |
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| 1:20 |
Average % |
87 |
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| Range % |
83-96 |
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| 1:40 |
Average % |
94 |
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| Range % |
90-100 |
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| 1:80 |
Average % |
94 |
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| Range % |
86-98 |
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回收率
| The recovery of human MYO/MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
97 |
90-110 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.175 |
2.211 |
2.193 |
2.107 |
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| 50 |
1.613 |
1.656 |
1.635 |
1.549 |
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| 25 |
1.024 |
1.004 |
1.014 |
0.928 |
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| 12.5 |
0.571 |
0.584 |
0.578 |
0.492 |
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| 6.25 |
0.312 |
0.308 |
0.310 |
0.224 |
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| 3.12 |
0.188 |
0.195 |
0.192 |
0.106 |
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| 1.56 |
0.129 |
0.125 |
0.127 |
0.041 |
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| 0 |
0.085 |
0.087 |
0.086 |
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货期
3-5 working days