产品描述
This Rat IL6R ELISA Kit was designed for the quantitative measurement of Rat IL6R protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
Il6r; Il6ra; Interleukin-6 receptor subunit alpha; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; CD antigen CD126
缩写
IL6R
Uniprot No
P22273
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-6R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 99 | |
| Range % | 91-103 | |
| 1:2 | Average % | 100 | |
| Range % | 95-104 | |
| 1:4 | Average % | 89 | |
| Range % | 83-92 | |
| 1:8 | Average % | 93 | |
| Range % | 84-97 | |
回收率
| The recovery of rat IL-6R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 101 | 92-105 | |
| EDTA plasma (n=4) | 99 | 91-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.492 | 2.437 | 2.465 | 2.289 | | | 20 | 2.077 | 1.981 | 2.029 | 1.853 | | | 10 | 1.289 | 1.300 | 1.295 | 1.119 | | | 5 | 0.799 | 0.787 | 0.793 | 0.617 | | | 2.5 | 0.470 | 0.461 | 0.466 | 0.290 | | | 1.25 | 0.394 | 0.388 | 0.391 | 0.215 | | | 0.625 | 0.260 | 0.252 | 0.256 | 0.080 | | | 0 | 0.178 | 0.174 | 0.176 | | |
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货期
3-5 working days