产品描述

This Human LGALS3BP ELISA Kit was designed for the quantitative measurement of Human LGALS3BP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

别名

90K ELISA Kit; 90K MAC 2 BP ELISA Kit; Basement membrane autoantigen p105 ELISA Kit; BTBD17B ELISA Kit; Galectin 3 binding protein ELISA Kit; Galectin-3-binding protein ELISA Kit; L3 antigen ELISA Kit; Lectin galactoside binding soluble 3 binding protein ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; LG3BP_HUMAN ELISA Kit; LGALS3BP ELISA Kit; M2BP ELISA Kit; Mac 2 binding protein ELISA Kit; Mac 2 BP ELISA Kit; Mac-2 BP ELISA Kit; Mac-2-binding protein ELISA Kit; Mac2 BP ELISA Kit; MAC2BP ELISA Kit; Serum protein 90K ELISA Kit; TANGO10B ELISA Kit; Transport and golgi organization 10 homolog B ELISA Kit; Tumor associated antigen 90K ELISA Kit; Tumor-associated antigen 90K ELISA Kit

缩写

LGALS3BP

Uniprot No

Q08380

灵敏度

0.39 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:20		Average %		91
		Range %		84-98
1:40		Average %		90
		Range %		85-95
1:60		Average %		97
		Range %		92-102
1:160		Average %		102
		Range %		96-108

回收率

The recovery of human LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		95-106
EDTA plasma (n=4)		90		85-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   100 2.620 2.645 2.633 2.541   50 2.265 2.366 2.316 2.224   25 1.628 1.673 1.651 1.559   12.5 0.921 0.855 0.888 0.796   6.25 0.571 0.589 0.580 0.488   3.12 0.333 0.355 0.344 0.252   1.56 0.190 0.188 0.189 0.097   0 0.093 0.090 0.092

货期

3-5 working days