产品描述
The human IL-25 ELISA kit is suitable for the quantitative determination of human IL-25 in different sample types, including serum, cell culturesupernates, urine, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
IL-25, generated by epithelial cells along with numerous innate immune cells, plays an important role in increasing Th2 cytokine synthesis. Once secreted, IL-25 can bind to the receptors, IL-17RA and IL-17RB, and activate the NF-κB pathway where it plays the role of a Th2 cell-driving cytokine. It participates in type 2 responses by promoting the production of Th2 cytokines and eosinophil infiltration. IL-25 is a major regulator that mediates innate and adaptive through the activation of ILC2s and Th2 cells to stimulate sustained Th2-skewed mucosal inflammation. Additionally, IL-25 has anti-inflammatory effects in autoimmune and inflammatory diseases via the downregulation of Th1 and Th17 cell responses.
别名
IL 17E ELISA Kit; IL 25 ELISA Kit; IL-17E ELISA Kit; IL-25 ELISA Kit; IL17e ELISA Kit; IL25 ELISA Kit; IL25_HUMAN ELISA Kit; Interleukin 17E ELISA Kit; Interleukin 25 ELISA Kit; Interleukin-17E ELISA Kit; Interleukin-25 ELISA Kit; Interleukin17E ELISA Kit; Interleukin25 ELISA Kit; OTTHUMP00000027946 ELISA Kit; OTTHUMP00000246238 ELISA Kit; UNQ3120/PRO10272 ELISA Kit
缩写
IL25
Uniprot No
Q9H293
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 87-98 | |
| 1:2 | Average % | 97 | |
| Range % | 90-103 | |
| 1:4 | Average % | 97 | |
| Range % | 90-106 | |
| 1:8 | Average % | 98 | |
| Range % | 94-103 | |
回收率
| The recovery of human IL-25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 84 | 80-89 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.892 | 2.752 | 2.822 | 2.711 | | | 2000 | 2.299 | 2.179 | 2.239 | 2.128 | | | 1000 | 1.645 | 1.599 | 1.622 | 1.511 | | | 500 | 0.982 | 1.002 | 0.992 | 0.881 | | | 250 | 0.561 | 0.602 | 0.582 | 0.471 | | | 125 | 0.350 | 0.366 | 0.358 | 0.247 | | | 62.5 | 0.268 | 0.275 | 0.272 | 0.161 | | | 0 | 0.109 | 0.112 | 0.111 | | |
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货期
3-5 working days
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