产品描述
INS(胰岛素)是一种多肽激素,由胰岛β细胞分泌,具有降低血糖的作用。胰岛素通过结合细胞膜上的胰岛素受体来调节糖代谢、脂肪代谢和蛋白质代谢。
华美生物所提供的Mouse Insulin,INS ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆、细胞裂解液样本中的INS,其灵敏度为3.9 nIU/mL,检测范围为15.6 nIU/mL-1000 nIU/mL。
别名
Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
缩写
INS
Uniprot No
P01325
灵敏度
3.9 nIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
90 |
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| Range % |
84-95 |
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| 1:200 |
Average % |
93 |
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| Range % |
90-96 |
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| 1:400 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:800 |
Average % |
94 |
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| Range % |
87-99 |
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回收率
| The recovery of mouse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-102 |
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| EDTA plasma (n=4) |
89 |
82-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| nIU/ml. |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.546 |
2.598 |
2.572 |
2.396 |
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| 500 |
2.231 |
2.267 |
2.249 |
2.073 |
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| 250 |
1.745 |
1.769 |
1.757 |
1.581 |
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| 125 |
1.198 |
1.163 |
1.181 |
1.005 |
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| 62.5 |
0.648 |
0.628 |
0.638 |
0.462 |
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| 31.2 |
0.426 |
0.407 |
0.417 |
0.241 |
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| 15.6 |
0.302 |
0.326 |
0.314 |
0.138 |
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| 0 |
0.173 |
0.179 |
0.176 |
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货期
3-5 working days