产品描述
This Human IL-28B ELISA Kit was designed for the quantitative measurement of Human IL-28B protein in serum, plasma, cell culture supernats, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
别名
Cytokine ZCYTO22 ELISA Kit; IFN-lambda-3 ELISA Kit; IFN-lambda-4 ELISA Kit; IL-28B ELISA Kit; IL-28C ELISA Kit; IL28B ELISA Kit; IL28B_HUMAN ELISA Kit; IL28C ELISA Kit; Interferon lambda-3 ELISA Kit; Interferon lambda-4 ELISA Kit; interleukin 28B (interferon; lambda 3) ELISA Kit; Interleukin 28C ELISA Kit; Interleukin-28B ELISA Kit; Interleukin-28C ELISA Kit
缩写
IL-28B
Uniprot No
Q8IZI9
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-28B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-92 | |
| 1:2 | Average % | 96 | |
| Range % | 90-100 | |
| 1:4 | Average % | 100 | |
| Range % | 95-105 | |
| 1:8 | Average % | 96 | |
| Range % | 93-99 | |
回收率
| The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-98 | |
| EDTA plasma (n=4) | 100 | 95-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.224 | 2.239 | 2.232 | 2.047 | | | 50 | 1.509 | 1.534 | 1.522 | 1.337 | | | 25 | 1.023 | 1.036 | 1.030 | 0.845 | | | 12.5 | 0.688 | 0.699 | 0.694 | 0.509 | | | 6.25 | 0.474 | 0.490 | 0.482 | 0.297 | | | 3.12 | 0.387 | 0.374 | 0.381 | 0.196 | | | 1.56 | 0.281 | 0.297 | 0.289 | 0.104 | | | 0 | 0.184 | 0.186 | 0.185 | | |
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货期
3-5 working days