产品描述
This Human Orexin A ELISA Kit was designed for the quantitative measurement of Human Orexin A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
别名
Hcrt ELISA Kit; Hcrt1 ELISA Kit; Hcrt2 ELISA Kit; Hypocretin ELISA Kit; Hypocretin neuropeptide precursor ELISA Kit; Hypocretin-1 ELISA Kit; Hypocretin-2 ELISA Kit; OREX_HUMAN ELISA Kit; Orexin ELISA Kit; Orexin precursor ELISA Kit; Orexin-B ELISA Kit; OX ELISA Kit; PPOX ELISA Kit
缩写
Orexin A
Uniprot No
O43612
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-92 | |
| 1:2 | Average % | 92 | |
| Range % | 87-96 | |
| 1:4 | Average % | 84 | |
| Range % | 81-86 | |
| 1:8 | Average % | 102 | |
| Range % | 100-106 | |
回收率
| The recovery of human orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-96 | |
| EDTA plasma (n=4) | 97 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.334 | 2.556 | 2.445 | 2.341 | | | 5 | 2.161 | 2.175 | 2.168 | 2.064 | | | 2.5 | 1.645 | 1.756 | 1.701 | 1.597 | | | 1.25 | 1.257 | 1.267 | 1.262 | 1.158 | | | 0.625 | 0.818 | 0.886 | 0.852 | 0.748 | | | 0.312 | 0.547 | 0.568 | 0.558 | 0.454 | | | 0.156 | 0.331 | 0.356 | 0.344 | 0.240 | | | 0 | 0.102 | 0.106 | 0.104 | | |
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货期
3-5 working days