产品描述
This Human MMP13 ELISA Kit was designed for the quantitative measurement of Human MMP13 protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
CLG 3 ELISA Kit; CLG3 ELISA Kit; Collagenase 3 ELISA Kit; Collagenase3 ELISA Kit; MANDP1 ELISA Kit; Matrix metallopeptidase 13 (collagenase 3) ELISA Kit; Matrix Metalloproteinase 13 ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP 13 ELISA Kit; MMP-13 ELISA Kit; Mmp13 ELISA Kit; MMP13_HUMAN ELISA Kit
缩写
MMP13
Uniprot No
P45452
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-99 | |
| 1:2 | Average % | 95 | |
| Range % | 92-99 | |
| 1:4 | Average % | 89 | |
| Range % | 82-95 | |
| 1:8 | Average % | 90 | |
| Range % | 84-97 | |
回收率
| The recovery of human MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 91-103 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.642 | 2.842 | 2.742 | 2.638 | | | 5 | 2.158 | 2.345 | 2.252 | 2.148 | | | 2.5 | 1.602 | 1.589 | 1.596 | 1.492 | | | 1.25 | 0.924 | 0.944 | 0.934 | 0.830 | | | 0.625 | 0.544 | 0.587 | 0.566 | 0.462 | | | 0.312 | 0.325 | 0.346 | 0.336 | 0.232 | | | 0.156 | 0.246 | 0.250 | 0.248 | 0.144 | | | 0 | 0.107 | 0.101 | 0.104 | | | | | | | | | | |
|
货期
3-5 working days