产品描述
This Dog IL6 ELISA Kit was designed for the quantitative measurement of Dog IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
别名
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL6
Uniprot No
P41323
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-88 | |
| 1:2 | Average % | 95 | |
| Range % | 89-105 | |
| 1:4 | Average % | 100 | |
| Range % | 95-106 | |
| 1:8 | Average % | 93 | |
| Range % | 89-97 | |
回收率
| The recovery of dog IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 82-96 | |
| EDTA plasma (n=4) | 93 | 85-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.954 | 2.989 | 2.972 | 2.855 | | | 50 | 2.388 | 2.386 | 2.387 | 2.270 | | | 25 | 1.758 | 1.796 | 1.777 | 1.660 | | | 12.5 | 1.035 | 1.106 | 1.071 | 0.954 | | | 6.25 | 0.502 | 0.513 | 0.508 | 0.391 | | | 3.12 | 0.271 | 0.268 | 0.270 | 0.153 | | | 1.56 | 0.184 | 0.196 | 0.190 | 0.073 | | | 0 | 0.112 | 0.121 | 0.117 | | |
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货期
3-5 working days