产品描述
This Fish IL1B ELISA Kit was designed for the quantitative measurement of Fish IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
il1bInterleukin-1 beta ELISA Kit
缩写
IL-1β
Uniprot No
Q9YGD3
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 85 | | |
| Range % | 82-89 | | |
| 1:2 | Average % | 94 | | |
| Range % | 90-99 | | |
| 1:4 | Average % | 104 | | |
| Range % | 97-108 | | |
| 1:8 | Average % | 99 | | |
| Range % | 94-106 | | |
回收率
| The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 89-102 | | |
| EDTA plasma (n=4) | 89 | 82-95 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 2.989 | 2.778 | 2.884 | 2.776 | | | | 100 | 2.467 | 2.432 | 2.450 | 2.342 | | | | 50 | 1.774 | 1.866 | 1.820 | 1.712 | | | | 25 | 0.956 | 0.932 | 0.944 | 0.836 | | | | 12.5 | 0.436 | 0.456 | 0.446 | 0.338 | | | | 6.25 | 0.258 | 0.270 | 0.264 | 0.156 | | | | 3.12 | 0.156 | 0.155 | 0.156 | 0.048 | | | | 0 | 0.105 | 0.111 | 0.108 | | | |
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货期
3-5 working days