产品描述
The human Mu-type opioid receptor (OPRM1) Elisa kit is suitable for quantitatively measuring human OPRM1 in serum, plasma, cell lysates, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of OPRM1 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, and lot-to-lot consistency.
OPRM1 is the primary site of action for the most commonly used opioids including morphine, heroin, and fentanyl and regulates reward derived from both drug use and natural experiences, including social interaction, through actions in the nucleus accumbens. Dysregulation of OPRM1 signaling may contribute to deficits in social interaction and other motivated behaviors that are a hallmark of neuropsychiatric disorders.
别名
OPRM1; MOR1; Mu-type opioid receptor; M-OR-1; MOR-1; Mu opiate receptor; Mu opioid receptor; MOP; hMOP
缩写
OPRM1
Uniprot No
P35372
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human OPRM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 85-101 | |
| 1:2 | Average % | 95 | |
| Range % | 89-99 | |
| 1:4 | Average % | 93 | |
| Range % | 88-103 | |
| 1:8 | Average % | 96 | |
| Range % | 83-105 | |
回收率
| The recovery of human OPRM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 85-99 | |
| EDTA plasma (n=4) | 98 | 95-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.123 | 2.091 | 2.107 | 2.003 | | | 1000 | 1.869 | 1.853 | 1.861 | 1.757 | | | 500 | 1.541 | 1.528 | 1.535 | 1.431 | | | 250 | 1.112 | 1.096 | 1.104 | 1.000 | | | 125 | 0.708 | 0.695 | 0.702 | 0.598 | | | 62.5 | 0.500 | 0.487 | 0.494 | 0.390 | | | 31.25 | 0.289 | 0.279 | 0.284 | 0.180 | | | 0 | 0.104 | 0.103 | 0.104 | | |
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货期
3-5 working days
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