产品描述
This Human LDHD ELISA Kit was designed for the quantitative measurement of Human LDHD protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 mIU/mL-2000 mIU/mL and the sensitivity is 7.8 mIU/mL.
别名
D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
缩写
LDHD
Uniprot No
Q86WU2
灵敏度
7.8 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 87-103 | |
| 1:2 | Average % | 91 | |
| Range % | 86-103 | |
| 1:4 | Average % | 94 | |
| Range % | 86-105 | |
| 1:8 | Average % | 91 | |
| Range % | 84-100 | |
回收率
| The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 91-106 | |
| EDTA plasma (n=4) | 96 | 86-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mIU/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.418 | 2.385 | 2.402 | 2.265 | | | 1000 | 1.773 | 1.728 | 1.751 | 1.614 | | | 500 | 0.936 | 1.002 | 0.969 | 0.832 | | | 250 | 0.624 | 0.651 | 0.638 | 0.501 | | | 125 | 0.407 | 0.422 | 0.415 | 0.278 | | | 62.5 | 0.282 | 0.287 | 0.285 | 0.148 | | | 31.25 | 0.253 | 0.264 | 0.259 | 0.122 | | | 0 | 0.135 | 0.139 | 0.137 | | |
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货期
3-5 working days