产品描述
This Rat MMP7 ELISA Kit was designed for the quantitative measurement of Rat MMP7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
Mmp7 ELISA Kit; Mmp-7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
缩写
MMP7
Uniprot No
P50280
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 88-98 | |
| 1:2 | Average % | 84 | |
| Range % | 81-88 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 97 | |
| Range % | 92-102 | |
回收率
| The recovery of rat MMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-100 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.612 | 2.637 | 2.625 | 2.470 | | | 500 | 1.792 | 1.736 | 1.764 | 1.609 | | | 250 | 1.101 | 1.074 | 1.088 | 0.933 | | | 125 | 0.603 | 0.634 | 0.619 | 0.464 | | | 62.5 | 0.431 | 0.414 | 0.423 | 0.268 | | | 31.25 | 0.333 | 0.309 | 0.321 | 0.166 | | | 15.6 | 0.232 | 0.219 | 0.226 | 0.071 | | | 0 | 0.156 | 0.153 | 0.155 | | |
|
货期
3-5 working days