产品描述
This Dog IL18 ELISA Kit was designed for the quantitative measurement of Dog IL18 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
别名
IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写
IL18
Uniprot No
Q9XSR0
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 104 | |
| Range % | 100-109 | |
| 1:2 | Average % | 97 | |
| Range % | 91-105 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 87 | 80-91 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.675 | 2.552 | 2.614 | 2.439 | | | 400 | 2.065 | 2.076 | 2.071 | 1.896 | | | 200 | 1.372 | 1.424 | 1.398 | 1.223 | | | 100 | 0.726 | 0.739 | 0.733 | 0.558 | | | 50 | 0.471 | 0.504 | 0.488 | 0.313 | | | 25 | 0.380 | 0.359 | 0.370 | 0.195 | | | 12.5 | 0.271 | 0.259 | 0.265 | 0.090 | | | 0 | 0.173 | 0.177 | 0.175 | | |
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货期
3-5 working days