产品描述

This Human KRT18 ELISA Kit was designed for the quantitative measurement of Human KRT18 protein in serum, plasma, tissue homogenates, cell lysates, cell_x005f_x000D_ culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-15 ng/mL and the sensitivity is 0.25 ng/mL.

别名

Cell proliferation inducing gene 46 protein ELISA Kit; Cell proliferation inducing protein 46 ELISA Kit; Cell proliferation-inducing gene 46 protein ELISA Kit; CK 18 ELISA Kit; CK-18 ELISA Kit; CK18 ELISA Kit; CYK 18 ELISA Kit; CYK18 ELISA Kit; Cytokeratin 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Cytokeratin-18 ELISA Kit; K 18 ELISA Kit; K18 ELISA Kit; K1C18_HUMAN ELISA Kit; KA18 ELISA Kit; Keratin 18 ELISA Kit; Keratin 18, type I ELISA Kit; Keratin D ELISA Kit; keratin, type I cytoskeletal 18 ELISA Kit; Keratin-18 ELISA Kit; Krt18 ELISA Kit

缩写

KRT18

Uniprot No

P05783

灵敏度

0.25 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		90-100
1:2		Average %		92
		Range %		88-99
1:4		Average %		97
		Range %		92-106
1:8		Average %		94
		Range %		86-103

回收率

The recovery of human CK-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-102
EDTA plasma (n=4)		97		92-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   15 1.932 1.958 1.945 1.898   7.5 1.318 1.357 1.338 1.291   3.75 0.786 0.798 0.792 0.745   1.25 0.375 0.362 0.369 0.322   0.625 0.216 0.223 0.220 0.173   0 0.046 0.047 0.047

货期

3-5 working days