产品描述
This Sheep IL2 ELISA Kit was designed for the quantitative measurement of Sheep IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写
IL2
Uniprot No
P19114
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 84-93 | |
| 1:2 | Average % | 98 | |
| Range % | 91-105 | |
| 1:4 | Average % | 97 | |
| Range % | 95-103 | |
| 1:8 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of sheep IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 96 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.418 | 2.425 | 2.422 | 2.335 | | | 1000 | 1.547 | 1.556 | 1.552 | 1.465 | | | 500 | 1.085 | 1.063 | 1.074 | 0.987 | | | 250 | 0.677 | 0.658 | 0.668 | 0.581 | | | 125 | 0.436 | 0.407 | 0.422 | 0.335 | | | 62.5 | 0.240 | 0.234 | 0.237 | 0.150 | | | 31.25 | 0.162 | 0.159 | 0.161 | 0.074 | | | 0 | 0.088 | 0.086 | 0.087 | | |
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货期
3-5 working days