产品描述

This Human MX1 ELISA Kit was designed for the quantitative measurement of Human MX1 protein in serum, plasma, urine, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

Human interferon regulated resistance GTP binding protein MXA ELISA Kit; IFI 78 ELISA Kit; IFI 78K ELISA Kit; IFI-78K ELISA Kit; IFI78 ELISA Kit; IFI78K ELISA Kit; Interferon induced GTP binding protein Mx1 ELISA Kit; Interferon induced protein p78 ELISA Kit; Interferon inducible protein p78 ELISA Kit; Interferon regulated resistance GTP binding protein ELISA Kit; Interferon regulated resistance GTP binding protein MxA ELISA Kit; Interferon-induced GTP-binding protein Mx1 ELISA Kit; Interferon-induced protein p78 ELISA Kit; Interferon-regulated resistance GTP-binding protein MxA ELISA Kit; MX 1 ELISA Kit; MX ELISA Kit; MX dynamin-like GTPase 1 ELISA Kit; MX1 ELISA Kit; MX1_HUMAN ELISA Kit; MxA ELISA Kit; Myxoma resistance protein 1 ELISA Kit; Myxovirus (influenza virus) resistance 1 interferon inducible ELISA Kit; Myxovirus (influenza virus) resistance 1 interferon inducible protein p78 ELISA Kit; Myxovirus (influenza) resistance 1 homolog of murine ELISA Kit; Myxovirus resistance 1; mouse; homolog of ELISA Kit; Myxovirus resistance protein 1 ELISA Kit; N-terminally processed ELISA Kit

缩写

MX1

Uniprot No

P20591

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-89
1:2		Average %		94
		Range %		91-99
1:4		Average %		95
		Range %		92-98
1:8		Average %		106
		Range %		98-110

回收率

The recovery of human MX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-99
EDTA plasma (n=4)		98		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 1.918 1.802 1.860 1.769 500 1.226 1.156 1.191 1.100 250 0.694 0.664 0.679 0.588 125 0.412 0.398 0.405 0.314 62.5 0.255 0.250 0.253 0.162 31.25 0.173 0.169 0.171 0.080 15.6 0.123 0.122 0.123 0.032 0 0.091 0.090 0.091

货期

3-5 working days