产品描述

This Human NPM1 ELISA Kit was designed for the quantitative measurement of Human NPM1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

B23 ELISA Kit; MGC104254 ELISA Kit; NO38 ELISA Kit; NPM ELISA Kit; NPM_HUMAN ELISA Kit; NPM1 ELISA Kit; Nucleolar phosphoprotein B23 ELISA Kit; Nucleolar protein NO38 ELISA Kit; Nucleophosmin (nucleolar phosphoprotein B23 numatrin) ELISA Kit; Nucleophosmin ELISA Kit; nucleophosmin nucleoplasmin family member 1 ELISA Kit; Nucleophosmin/nucleoplasmin family member 1 ELISA Kit; Numatrin ELISA Kit; OTTHUMP00000161024 ELISA Kit; OTTHUMP00000161025 ELISA Kit; OTTHUMP00000223397 ELISA Kit; OTTHUMP00000223398 ELISA Kit

缩写

NPM1

Uniprot No

P06748

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NPM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		87-95
1:2		Average %		96
		Range %		90-102
1:4		Average %		97
		Range %		94-101
1:8		Average %		95
		Range %		90-98

回收率

The recovery of human NPM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-96
EDTA plasma (n=4)		97		93-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.389 2.380 2.385 2.239 750 1.604 1.545 1.575 1.429 375 1.122 1.068 1.095 0.949 187.5 0.721 0.733 0.727 0.581 94 0.424 0.390 0.407 0.261 47 0.303 0.301 0.302 0.156 23.5 0.230 0.237 0.234 0.088 0 0.141 0.150 0.146

货期

3-5 working days