产品描述
This Rat IL1RN ELISA Kit was designed for the quantitative measurement of Rat IL1RN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .
别名
Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit
缩写
IL1RN
Uniprot No
P25086
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 102 | |
| Range % | 98-105 | |
| 1:2 | Average % | 97 | |
| Range % | 92-102 | |
| 1:4 | Average % | 96 | |
| Range % | 90-101 | |
| 1:8 | Average % | 93 | |
| Range % | 88-99 | |
回收率
| The recovery of rat IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 92-104 | |
| EDTA plasma (n=4) | 89 | 82-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.259 | 2.269 | 2.264 | 2.148 | | | 4000 | 1.554 | 1.597 | 1.576 | 1.460 | | | 2000 | 1.040 | 1.068 | 1.054 | 0.938 | | | 1000 | 0.625 | 0.672 | 0.649 | 0.533 | | | 500 | 0.398 | 0.371 | 0.385 | 0.269 | | | 250 | 0.279 | 0.274 | 0.277 | 0.161 | | | 125 | 0.181 | 0.189 | 0.185 | 0.069 | | | 0 | 0.114 | 0.118 | 0.116 | | |
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货期
3-5 working days