产品描述
This Mouse IL12B ELISA Kit was designed for the quantitative measurement of Mouse IL12B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
Il12bInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写
IL12B
Uniprot No
P43432
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 92 | |
| Range % | 88-95 | |
| 1:10 | Average % | 90 | |
| Range % | 85-92 | |
| 1:20 | Average % | 89 | |
| Range % | 84-96 | |
| 1:40 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of mouse IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-100 | |
| EDTA plasma (n=4) | 93 | 87-99 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.701 | 2.852 | 2.777 | 2.633 | | | 500 | 2.005 | 2.076 | 2.041 | 1.897 | | | 250 | 1.248 | 1.316 | 1.282 | 1.138 | | | 125 | 0.754 | 0.704 | 0.729 | 0.585 | | | 62.5 | 0.476 | 0.450 | 0.463 | 0.319 | | | 31.2 | 0.311 | 0.323 | 0.317 | 0.173 | | | 15.6 | 0.252 | 0.259 | 0.256 | 0.112 | | | 0 | 0.148 | 0.139 | 0.144 | | |
|
货期
3-5 working days