产品描述

The Human 8-oxoguanine DNA glycosydase (hOGG1) ELISA kit is designed to enable accurate and reliable detection of 8-oxoguanine DNA glycosydase in Homo sapiens (Human) samples.

This ELISA kit offers a versatile solution for researchers studying cancer、DNA repair, allowing for the detection of hOGG1 in serum, tissue homogenates, and cell lysates. With a wide detection range of 0.312 ng/mL-20 ng/mL and a sensitivity of 0.078 ng/mL, this kit provides accurate and sensitive measurements of hOGG1 levels in samples.

The assay time for this ELISA kit is 1-5 hours, and sample volume ranges from 50-100ul. The detection wavelength is 450 nm, and the measurement method is sandwich, providing a quantitative approach for researchers studying the role of hOGG1 in cancer and DNA repair

别名

8 hydroxyguanine DNA glycosylase ELISA Kit; 8 oxoguanine DNA glycosylase 1 ELISA Kit; 8 oxoguanine DNA glycosylase ELISA Kit; AP lyase ELISA Kit; DNA (apurinic or apyrimidinic site) lyase ELISA Kit; DNA apurinic or apyrimidinic site lyase ELISA Kit; DNA lyase ELISA Kit; DNA-(apurinic or apyrimidinic site) lyase ELISA Kit; HMMH ELISA Kit; HOGG 1 ELISA Kit; HOGG1 ELISA Kit; MMH ELISA Kit; MUTM ELISA Kit; N glycosylase ELISA Kit; Ogg 1 ELISA Kit; Ogg1 ELISA Kit; OGG1_HUMAN ELISA Kit; OGH 1 ELISA Kit; OGH1 ELISA Kit

缩写

OGG1

Uniprot No

O15527

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human hOGG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		103
		Range %		98-112
1:2		Average %		84
		Range %		80-88
1:4		Average %		92
		Range %		88-96
1:8		Average %		90
		Range %		85-94

回收率

The recovery of human hOGG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		92-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.737 2.872 2.805 2.654 10 2.096 2.115 2.106 1.955 5 1.483 1.496 1.490 1.339 2.5 0.872 0.839 0.856 0.705 1.25 0.561 0.524 0.543 0.392 0.625 0.348 0.332 0.340 0.189 0.312 0.257 0.261 0.259 0.108 0 0.145 0.157 0.151

货期

3-5 working days