产品描述

This Human LGALS1 ELISA Kit was designed for the quantitative measurement of Human LGALS1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

别名

14 kDa laminin-binding protein ELISA Kit; 14 kDa lectin ELISA Kit; Beta galactoside binding lectin ELISA Kit; Beta galactoside binding lectin L 14 I ELISA Kit; beta galactoside binding protein ELISA Kit; Beta-galactoside-binding lectin L-14-I ELISA Kit; DKFZp686E23103 ELISA Kit; Gal 1 ELISA Kit; Gal-1 ELISA Kit; GAL1 ELISA Kit; Galaptin ELISA Kit; Galbp ELISA Kit; Galectin ELISA Kit; Galectin-1 ELISA Kit; Galectin1 ELISA Kit; GBP ELISA Kit; HBL ELISA Kit; HLBP14 ELISA Kit; HPL ELISA Kit; L 14.5 ELISA Kit; L-14.5 ELISA Kit; L14 ELISA Kit; Lactose binding lectin 1 ELISA Kit; Lactose-binding lectin 1 ELISA Kit; Lect14 ELISA Kit; Lectin galactoside binding soluble 1 ELISA Kit; Lectin galactoside-binding soluble 1 ELISA Kit; LEG1_HUMAN ELISA Kit; LGALS 1 ELISA Kit; LGALS1 ELISA Kit; Lgals1 lectin galactose binding soluble 1 ELISA Kit; MAPK activating protein MP12 ELISA Kit; Putative MAPK activating protein MP12 ELISA Kit; Putative MAPK-activating protein PM12 ELISA Kit; S Lac lectin 1 ELISA Kit; S-Lac lectin 1 ELISA Kit

缩写

LGALS1

Uniprot No

P09382

灵敏度

0.78 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		80-94
1:2		Average %		85
		Range %		80-92
1:4		Average %		92
		Range %		87-99
1:8		Average %		97
		Range %		90-105

回收率

The recovery of human LGALS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		83-98
EDTA plasma (n=4)		94		88-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   200 2.312 2.143 2.228 2.074   100 1.953 1.823 1.888 1.734   50 1.562 1.482 1.522 1.368   25 1.198 1.134 1.166 1.012   12.5 0.647 0.596 0.622 0.468   6.25 0.371 0.347 0.359 0.205   3.12 0.234 0.219 0.227 0.073   0 0.158 0.149 0.154

货期

3-5 working days