产品描述

This Human KI-67 ELISA Kit was designed for the quantitative measurement of Human KI-67 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

Antigen identified by monoclonal ELISA Kit Ki 67 ELISA Kit; Antigen identified by monoclonal ELISA Kit Ki-67 ELISA Kit; Antigen KI-67 ELISA Kit; Antigen KI67 ELISA Kit; Antigen Ki67 ELISA Kit; KI67_HUMAN ELISA Kit; KIA ELISA Kit; Marker of proliferation Ki-67 ELISA Kit; MIB 1 ELISA Kit; MIB ELISA Kit; MKI67 ELISA Kit; PPP1R105 ELISA Kit; Proliferation marker protein Ki-67 ELISA Kit; Proliferation related Ki 67 antigen ELISA Kit; Protein phosphatase 1 regulatory subunit 105 ELISA Kit; RP11-380J17.2 ELISA Kit

缩写

MKI67

Uniprot No

P46013

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Ki-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		86-106
1:2		Average %		97
		Range %		91-105
1:4		Average %		92
		Range %		87-98
1:8		Average %		99
		Range %		93-107

回收率

The recovery of human Ki-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-106
EDTA plasma (n=4)		99		95-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.243 2.281 2.262 2.070 10 1.644 1.651 1.648 1.456 5 1.177 1.271 1.224 1.032 2.5 0.874 0.899 0.887 0.695 1.25 0.613 0.627 0.620 0.428 0.625 0.468 0.478 0.473 0.281 0.312 0.373 0.389 0.381 0.189 0 0.191 0.193 0.192

货期

3-5 working days