产品描述
This Pig IL1A ELISA Kit was designed for the quantitative measurement of Pig IL1A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.
别名
IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写
IL1A
Uniprot No
P18430
灵敏度
6.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-93 | |
| 1:2 | Average % | 104 | |
| Range % | 100-108 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 87 | |
| Range % | 83-92 | |
回收率
| The recovery of pig IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 95-103 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1600 | 2.599 | 2.535 | 2.567 | 2.385 | | | 800 | 1.839 | 1.899 | 1.869 | 1.687 | | | 400 | 1.174 | 1.197 | 1.186 | 1.004 | | | 200 | 0.753 | 0.742 | 0.748 | 0.566 | | | 100 | 0.460 | 0.499 | 0.480 | 0.298 | | | 50 | 0.368 | 0.372 | 0.370 | 0.188 | | | 25 | 0.284 | 0.292 | 0.288 | 0.106 | | | 0 | 0.181 | 0.183 | 0.182 | | |
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货期
3-5 working days