产品描述
This Mouse NT-3 ELISA Kit was designed for the quantitative measurement of Mouse NT-3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit
缩写
NT-3
Uniprot No
P20181
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 105 | | |
| Range % | 87-109 | | |
| 1:2 | Average % | 102 | | |
| Range % | 97-106 | | |
| 1:4 | Average % | 97 | | |
| Range % | 90-100 | | |
| 1:8 | Average % | 98 | | |
| Range % | 91-102 | | |
回收率
| The recovery of mouse NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 111 | 100-116 | | |
| EDTA plasma (n=4) | 100 | 90-104 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 4000 | 2.179 | 2.107 | 2.143 | 1.977 | | | | 2000 | 1.533 | 1.578 | 1.556 | 1.390 | | | | 1000 | 1.041 | 1.073 | 1.057 | 0.891 | | | | 500 | 0.655 | 0.678 | 0.667 | 0.501 | | | | 250 | 0.487 | 0.500 | 0.494 | 0.328 | | | | 125 | 0.401 | 0.415 | 0.408 | 0.242 | | | | 62.5 | 0.278 | 0.284 | 0.281 | 0.115 | | | | 0 | 0.168 | 0.164 | 0.166 | | | |
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货期
3-5 working days