产品描述
The rat LDHA ELISA Kit is engineered for accurate measurement of rat LDHA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the LDHA content in the sample. The color intensity is positively correlated with LDHA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
LDHA is a cytosolic enzyme that catalyzes the conversion of pyruvate to lactate under anaerobic conditions. It is primarily found in skeletal muscle. LDHA is necessary to maintain glycolysis and ATP synthesis in the absence of sufficient oxygen by recycling NAD+ from NADH. LDHA also participates in the modulation of transcription by regulating the cellular redox state. During the differentiation of thymocytes, LDHA also functions as a molecular chaperone or as an association molecule. Even when oxygen is available, cancer cells employ LDHA to boost glycolysis, ATP, and lactate synthesis. Overexpression of LDHA has been associated with a number of additional unfavorable prognostic variables, including tumor hypoxia, angiogenesis, proliferation, and glucose uptake, as well as chemotherapy and radiotherapy resistance.
别名
Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
缩写
LDHA
Uniprot No
P04642
灵敏度
9.77 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 80-97 | |
| 1:2 | Average % | 92 | |
| Range % | 88-105 | |
| 1:4 | Average % | 97 | |
| Range % | 92-104 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-100 | |
| EDTA plasma (n=4) | 90 | 85-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | mU/ml | OD1 | OD2 | Average | Corrected | | | 2500 | 2.754 | 2.952 | 2.853 | 2.679 | | | 1250 | 2.218 | 2.376 | 2.297 | 2.123 | | | 625 | 1.534 | 1.584 | 1.559 | 1.385 | | | 312.5 | 1.022 | 1.039 | 1.031 | 0.857 | | | 156 | 0.587 | 0.624 | 0.606 | 0.432 | | | 78 | 0.412 | 0.430 | 0.421 | 0.247 | | | 39 | 0.286 | 0.279 | 0.283 | 0.109 | | | 0 | 0.173 | 0.175 | 0.174 | | |
|
货期
3-5 working days
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