产品描述

This Human KLK7 ELISA Kit was designed for the quantitative measurement of Human KLK7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

Chymotryptic stratum corneum ELISA Kit; hK 7 ELISA Kit; hK7 ELISA Kit; hSCCE ELISA Kit; Kallikrein 7 (chymotryptic stratum corneum) ELISA Kit; Kallikrein related peptidase 7 ELISA Kit; Kallikrein-7 ELISA Kit; Kallikrein7 ELISA Kit; KLK 7 ELISA Kit; Klk7 ELISA Kit; KLK7_HUMAN ELISA Kit; Protease serine 6 ELISA Kit; PRSS 6 ELISA Kit; PRSS6 ELISA Kit; SCCE ELISA Kit; Serine protease 6 ELISA Kit; Signal protein ELISA Kit; Stratum corneum chymotryptic enzyme ELISA Kit

缩写

KLK7

Uniprot No

P49862

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		102
		Range %		92-108
1:2		Average %		94
		Range %		91-97
1:4		Average %		92
		Range %		86-98
1:8		Average %		97
		Range %		92-103

回收率

The recovery of human KLK 7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		98-108
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.708 2.607 2.658 2.514 20 1.865 1.764 1.815 1.671 10 1.217 1.206 1.212 1.068 5 0.811 0.840 0.826 0.682 2.5 0.458 0.447 0.453 0.309 1.25 0.322 0.311 0.317 0.173 0.625 0.235 0.232 0.234 0.090 0 0.144 0.143 0.144

货期

3-5 working days