产品描述
This Chicken IL12/70 ELISA Kit was designed for the quantitative measurement of Chicken IL12/70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
缩写
IL-12/70
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
88-98 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:8 |
Average % |
96 |
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| Range % |
92-101 |
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回收率
| The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.192 |
0.188 |
0.190 |
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| 50 |
0.273 |
0.281 |
0.277 |
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| 12.5 |
0.585 |
0.565 |
0.575 |
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| 3.12 |
0.876 |
0.824 |
0.850 |
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| 0.78 |
1.424 |
1.457 |
1.441 |
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| 0 |
2.265 |
2.233 |
2.249 |
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货期
3-5 working days