产品描述
This Chicken IL-4 ELISA Kit was designed for the quantitative measurement of Chicken IL-4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
缩写
IL-4
Uniprot No
Q5W4U1
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 80-95 | |
| 1:2 | Average % | 94 | |
| Range % | 88-101 | |
| 1:4 | Average % | 93 | |
| Range % | 86-106 | |
| 1:8 | Average % | 95 | |
| Range % | 82-100 | |
回收率
| The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-97 | |
| EDTA plasma (n=4) | 92 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 1.726 | 1.825 | 1.776 | 1.665 | | | 400 | 1.336 | 1.383 | 1.360 | 1.249 | | | 200 | 1.060 | 0.981 | 1.021 | 0.910 | | | 100 | 0.714 | 0.706 | 0.710 | 0.599 | | | 50 | 0.481 | 0.475 | 0.478 | 0.367 | | | 25 | 0.307 | 0.292 | 0.300 | 0.189 | | | 12.5 | 0.227 | 0.214 | 0.221 | 0.110 | | | 0 | 0.108 | 0.113 | 0.111 | | |
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货期
3-5 working days