产品描述

This Human NME1 ELISA Kit was designed for the quantitative measurement of Human NME1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

别名

AWD ELISA Kit; AWD, drosophila, homolog of ELISA Kit; GAAD ELISA Kit; Granzyme A activated DNase ELISA Kit; Granzyme A-activated DNase ELISA Kit; GZMA activated DNase ELISA Kit; Metastasis inhibition factor NM23 ELISA Kit; NB ELISA Kit; NBS ELISA Kit; NDK A ELISA Kit; NDKA ELISA Kit; NDKA_HUMAN ELISA Kit; NDP kinase A ELISA Kit; NDPK-A ELISA Kit; NDPKA ELISA Kit; NM23 ELISA Kit; NM23 long variant, included ELISA Kit; nm23-H1 ELISA Kit; NM23-M1 ELISA Kit; NM23H1B, included ELISA Kit; NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit; Nme1 ELISA Kit; NME1-NME2 spliced read-through transcript, included ELISA Kit; Non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit; Nonmetastatic cells 1, protein expressed in ELISA Kit; Nonmetastatic protein 23 ELISA Kit; Nonmetastatic protein 23, homolog 1 ELISA Kit; Nucleoside diphosphate kinase A ELISA Kit; Tumor metastatic process-associated protein ELISA Kit

缩写

NME1

Uniprot No

P15531

灵敏度

4.68 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NME1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		96
		Range %		84-105
1:4		Average %		95
		Range %		90-100
1:8		Average %		92
		Range %		80-100

回收率

The recovery of human NME1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		81-98
EDTA plasma (n=4)		98		90-110

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1200 2.112 2.052 2.082 1.931 600 1.849 1.706 1.778 1.627 300 1.493 1.421 1.457 1.306 150 1.021 0.993 1.007 0.856 75 0.578 0.554 0.566 0.415 37.5 0.342 0.315 0.329 0.178 18.75 0.231 0.219 0.225 0.074 0 0.155 0.147 0.151

货期

3-5 working days