产品描述
This Mouse MMP13 ELISA Kit was designed for the quantitative measurement of Mouse MMP13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.438 pg/mL.
别名
Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
缩写
MMP13
Uniprot No
P33435
灵敏度
23.438 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 85-100 | |
| 1:2 | Average % | 92 | |
| Range % | 90-100 | |
| 1:4 | Average % | 96 | |
| Range % | 92-100 | |
| 1:8 | Average % | 93 | |
| Range % | 82-98 | |
回收率
| The recovery of mouse MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 81-98 | |
| EDTA plasma (n=4) | 94 | 92-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 6000 | 2.720 | 2.642 | 2.681 | 2.453 | | | 3000 | 2.416 | 2.381 | 2.399 | 2.171 | | | 1500 | 2.112 | 2.043 | 2.078 | 1.850 | | | 750 | 1.732 | 1.690 | 1.711 | 1.483 | | | 375 | 1.337 | 1.244 | 1.291 | 1.063 | | | 187.5 | 0.914 | 0.876 | 0.895 | 0.667 | | | 93.75 | 0.534 | 0.492 | 0.513 | 0.285 | | | 0 | 0.237 | 0.219 | 0.228 | | |
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货期
3-5 working days