产品描述
This Guinea pig IL5 ELISA Kit was designed for the quantitative measurement of Guinea pig IL5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写
IL5
Uniprot No
O08987
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 87-96 | |
| 1:2 | Average % | 94 | |
| Range % | 89-99 | |
| 1:4 | Average % | 103 | |
| Range % | 98-108 | |
| 1:8 | Average % | 100 | |
| Range % | 91-105 | |
回收率
| The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 92-97 | |
| EDTA plasma (n=4) | 92 | 84-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.516 | 2.489 | 2.503 | 2.391 | | | 1000 | 1.384 | 1.356 | 1.370 | 1.258 | | | 500 | 0.733 | 0.698 | 0.716 | 0.604 | | | 250 | 0.384 | 0.375 | 0.380 | 0.268 | | | 125 | 0.253 | 0.234 | 0.244 | 0.132 | | | 62.5 | 0.187 | 0.181 | 0.184 | 0.072 | | | 31.25 | 0.142 | 0.133 | 0.138 | 0.026 | | | 0 | 0.113 | 0.111 | 0.112 | | |
|
货期
3-5 working days