产品描述
IgM是一种多聚体免疫球蛋白,通常作为初次感染后产生的第一个免疫球蛋白。IgM能够识别并结合细菌、病毒和其他外来物质,从而激活免疫系统并参与免疫反应。IgM的水平可以用于诊断某些疾病,如乙肝、风疹等。
华美生物所提供的Bovine Immunoglobulin M,IgM ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、细胞培养上清、组织匀浆样本中的IgM,其灵敏度为0.2 μg/mL,检测范围为0.39 μg/mL-25 μg/mL。
缩写
IgM
灵敏度
0.2 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
91 |
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| Range % |
86-99 |
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| 1:200 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:400 |
Average % |
85 |
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| Range % |
81-87 |
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| 1:800 |
Average % |
89 |
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| Range % |
83-95 |
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回收率
| The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-97 |
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| EDTA plasma (n=4) |
97 |
92-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 25 |
0.059 |
0.056 |
0.058 |
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| 12.5 |
0.091 |
0.099 |
0.095 |
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| 6.25 |
0.151 |
0.152 |
0.152 |
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| 3.12 |
0.312 |
0.292 |
0.302 |
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| 1.56 |
0.608 |
0.660 |
0.634 |
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| 0.78 |
0.824 |
0.801 |
0.813 |
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| 0.39 |
1.150 |
1.192 |
1.171 |
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| 0 |
1.552 |
1.508 |
1.530 |
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货期
3-5 working days