产品描述

This Human MYC ELISA Kit was designed for the quantitative measurement of Human MYC protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

AU016757 ELISA Kit; Avian myelocytomatosis viral oncogene homolog ELISA Kit; bHLHe39 ELISA Kit; c Myc ELISA Kit; Cellular myelocytomatosis oncogene ELISA Kit; Class E basic helix-loop-helix protein 39 ELISA Kit; MGC105490 ELISA Kit; MRTL ELISA Kit; Myc ELISA Kit; Myc protein ELISA Kit; Myc proto oncogene protein ELISA Kit; Myc proto-oncogene protein ELISA Kit; myc-related translation/localization regulatory factor ELISA Kit; MYC_HUMAN ELISA Kit; Myc2 ELISA Kit; myca ELISA Kit; MYCC ELISA Kit; Myelocytomatosis oncogene a ELISA Kit; Myelocytomatosis oncogene ELISA Kit; Niard ELISA Kit; Nird ELISA Kit; oncogene c-Myc ELISA Kit; Oncogene Myc ELISA Kit; OTTHUMP00000158589 ELISA Kit; OTTHUMP00000227763 ELISA Kit; Proto-oncogene c-Myc ELISA Kit; Protooncogene homologous to myelocytomatosis virus ELISA Kit; RNCMYC ELISA Kit; Transcription factor p64 ELISA Kit; Transcriptional regulator Myc-A ELISA Kit; V-Myc avian myelocytomatosis viral oncogene homolog ELISA Kit; v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit; zc-myc ELISA Kit

缩写

MYC

Uniprot No

P01106

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human c-myc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		87-99
1:2		Average %		95
		Range %		91-101
1:4		Average %		92
		Range %		88-96
1:8		Average %		90
		Range %		86-97

回收率

The recovery of human c-myc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-96
EDTA plasma (n=4)		92		87-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.404 2.453 2.429 2.341 10 1.587 1.575 1.581 1.493 5 1.084 1.066 1.075 0.987 2.5 0.658 0.699 0.679 0.591 1.25 0.456 0.487 0.472 0.384 0.625 0.251 0.245 0.248 0.160 0.312 0.161 0.169 0.165 0.077 0 0.087 0.089 0.088

货期

3-5 working days