产品描述
IGFBP3(Insulin-like growth factor-binding protein 3)是一种结合IGF-1和IGF-2的血浆载体蛋白,属于胰岛素样生长因子结合蛋白家族,广泛存在于人体各种组织和液体中,尤其是肝脏和成骨细胞。IGFBP3不仅参与胰岛素样生长因子信号传导,还参与一些重要的细胞生理过程,如细胞增殖、分化、凋亡等,具有很高的生物学意义。IGFBP3的含量变化在一些疾病的发生和发展中发挥重要作用,如肿瘤、糖尿病、心血管疾病等。
华美生物所提供的Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的IGFBP3,其灵敏度为0.195 ng/mL,检测范围为0.78 ng/mL-50 ng/mL。
别名
Acid stable subunit of the 140 K IGF complex ELISA Kit; Binding protein 29 ELISA Kit; Binding protein 53 ELISA Kit; BP 53 ELISA Kit; BP53 ELISA Kit; Growth hormone dependent binding protein ELISA Kit; IBP 3 ELISA Kit; IBP-3 ELISA Kit; IBP3 ELISA Kit; IBP3_HUMAN ELISA Kit; IGF binding protein 3 ELISA Kit; IGF-binding protein 3 ELISA Kit; IGFBP 3 ELISA Kit; IGFBP-3 ELISA Kit; IGFBP3 ELISA Kit; Insulin Like Growth Factor Binding Protein 3 ELISA Kit; Insulin-like growth factor binding protein 3 precursor ELISA Kit; Insulin-like growth factor-binding protein 3 ELISA Kit
缩写
IGFBP3
Uniprot No
P17936
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:10 |
Average % |
92 |
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| Range % |
82-103 |
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| 1:20 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:40 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:80 |
Average % |
91 |
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| Range % |
85-100 |
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回收率
| The recovery of human IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-106 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.119 |
2.119 |
2.119 |
1.957 |
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| 25 |
1.560 |
1.521 |
1.541 |
1.379 |
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| 12.5 |
1.012 |
1.080 |
1.046 |
0.884 |
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| 6.25 |
0.716 |
0.726 |
0.721 |
0.559 |
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| 3.12 |
0.471 |
0.454 |
0.463 |
0.301 |
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| 1.56 |
0.321 |
0.334 |
0.328 |
0.166 |
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| 0.78 |
0.235 |
0.243 |
0.239 |
0.077 |
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| 0 |
0.161 |
0.163 |
0.162 |
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货期
3-5 working days