产品描述
The Guinea pig interleukin 2 (IL-2) ELISA Kit is a powerful tool for researchers in the field of immunology. The kit is designed for quantitative measurement of interleukin-2 protein in samples such as serum, plasma, and tissue homogenates of Cavia porcellus (Guinea pig).
The kit offers a broad detection range of 31.25 pg/mL-2000 pg/mL and a high sensitivity of 7.8 pg/mL, enabling accurate and reliable measurement of IL-2 levels in even low-concentration samples. With an assay time of 1-5 hours and a sample volume requirement of only 50-100ul, our kit is efficient and easy to use.
This Guinea pig IL-2 ELISA Kit employs a sandwich assay principle, where the target IL-2 protein is captured by a specific antibody and detected by a second antibody conjugated to a substrate. And the detection wavelength is 450 nm.
缩写
IL-2
Uniprot No
H0VBY4
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 102 | |
| Range % | 98-108 | |
| 1:2 | Average % | 94 | |
| Range % | 91-98 | |
| 1:4 | Average % | 103 | |
| Range % | 97-108 | |
| 1:8 | Average % | 88 | |
| Range % | 80-96 | |
回收率
| The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 88-100 | |
| EDTA plasma (n=4) | 104 | 96-108 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.230 | 2.351 | 2.291 | 2.152 | | | 1000 | 1.713 | 1.794 | 1.754 | 1.615 | | | 500 | 1.214 | 1.235 | 1.225 | 1.086 | | | 250 | 0.743 | 0.732 | 0.738 | 0.599 | | | 125 | 0.495 | 0.464 | 0.480 | 0.341 | | | 62.5 | 0.349 | 0.338 | 0.344 | 0.205 | | | 31.25 | 0.255 | 0.251 | 0.253 | 0.114 | | | 0 | 0.139 | 0.138 | 0.139 | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
